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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-KARS antibody (500-597) (Clone: S4MR)

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Cat# NB-22-48763-200

Size : 200uL(2x100uL)

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Phone : +1 850 650 7790

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Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of extracts of various cell lines, using LysRS/KARS rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of U-2 OS cells using LysRS/KARS Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat testis using LysRS/KARS rabbit monoclonal antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using LysRS/KARS Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse spinal cord using LysRS/KARS rabbit monoclonal antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using LysRS/KARS Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using LysRS/KARS rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using LysRS/KARS Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using LysRS/KARS rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded rat testis using LysRS/KARS Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of U-2 OS cells using LysRS/KARS rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using LysRS/KARS Rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-LysRS (500-597) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	KARS1
Gene ID:	3735
Uniprot ID:	SYK_HUMAN
Immunogen Region:	500-597
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-597 of human LysRS/KARS (Q15046).
Immunogen Sequence:	TELNDPMRQRQLFEEQAKAK AAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLT DSNNIKEVLLFPAMKPEDKK ENVATTDTLESTTVGTSV

Description

Post Translational Modifications	Phosphorylated on a serine residue after mast cell stimulation with immunoglobulin E (IgE).
Function	Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity. (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Protein Name	Lysine--Trna Ligase Lysyl-Trna Synthetase Lysrs
Database Links	Reactome: R-HSA-2408522 Reactome: R-HSA-379716 Reactome: R-HSA-379726
Cellular Localisation	Isoform Cytoplasmic: Cytoplasm Cytosol Cytoplasm Nucleus Cell Membrane Peripheral Membrane Protein Secreted Secretion Is Induced By Tnf-Alpha Cytosolic In Quiescent Mast Cells Translocates Into The Nucleus In Response To Mast Cell Activation By Immunoglobulin E Isoform Mitochondrial: Mitochondrion
Alternative Antibody Names	Anti-Lysine--Trna Ligase antibody Anti-Lysyl-Trna Synthetase antibody Anti-Lysrs antibody Anti-KARS1 antibody Anti-KARS antibody Anti-KIAA0070 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance