Reagents and instruments for immunology, cell biology and molecular biology.

TNF Receptor Type II, soluble, recombinant Human

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Cat# NB-50-0384-500

Size : 500µg

Contact local distributor :

Phone : +1 850 650 7790

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Contact local distributor :

Phone : +1 850 650 7790

Product Details

310-12-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Human, Mouse, Rat

Expression System

E. coli

Amino acid sequence

MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP

Molecular weight

18.9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of Recombinant Human TNF-alpha, is 0.125 ug/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human sTNF Receptor Type II is an 18.9 kDa protein (174 amino acid residues) comprising the cysteine-rich, ligand binding portion of the extracellular domain of the TNFRII protein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Generation of a single-cell B cell atlas of antibody repertoires and transcriptomes to identify signatures associated with antigen specificity.

iScience

Agrafiotis A,Neumeier D,Hong KL,Chowdhury T,Ehling R,Kuhn R,Sandu I,Kreiner V,Cotet TS,Shlesinger D,Laslo D,Anzböck S,Starkie D,Lightwood DJ,Oxenius A,Reddy ST,Yermanos A

310-12 was used in In vivo experiments to determine the relationship between antibody repertoires, gene expression signatures, and antigen specificity across 100,000 B cells.

Fri Mar 17 00:00:00 EDT 2023

Generation of a single-cell B cell atlas of antibody repertoires and transcriptomes to identify signatures associated with antigen specificity.

iScience

Agrafiotis A,Neumeier D,Hong KL,Chowdhury T,Ehling R,Kuhn R,Sandu I,Kreiner V,Cotet TS,Shlesinger D,Laslo D,Anzböck S,Starkie D,Lightwood DJ,Oxenius A,Reddy ST,Yermanos A

310-12 was used in In vivo experiments to determine the relationship between antibody repertoires, gene expression signatures, and antigen specificity across 100,000 B cells.

Fri Mar 17 00:00:00 EDT 2023

Profiling the specificity of clonally expanded plasma cells during chronic viral infection by single-cell analysis.

European journal of immunology

Neumeier D,Pedrioli A,Genovese A,Sandu I,Ehling R,Hong KL,Papadopoulou C,Agrafiotis A,Kuhn R,Shlesinger D,Robbiani D,Han J,Hauri L,Csepregi L,Greiff V,Merkler D,Reddy ST,Oxenius A,Yermanos A