Aspartate aminotransferase (AST), also known as serum glutamic-oxaloacetic transaminase (SGOT), is a crucial enzyme involved in amino acid metabolism. It catalyzes the conversion of aspartate and α-ketoglutarate to oxaloacetate and glutamate. AST is widely distributed in tissues such as the liver, heart, skeletal muscle, and kidneys, making it a valuable marker for assessing liver function and diagnosing conditions affecting these tissues.

Key Features of AST Assay Kits

• Sensitivity and Detection Range: AST assay kits are designed to be highly sensitive allowing for precise measurement of AST activity in various biological samples.
• Sample Types: These kits can be used with a variety of sample types, including serum, plasma, cell lysates, and tissue extracts, making them versatile for different research applications.
• Assay Methods: The assays often involve colorimetric or fluorometric detection methods. Colorimetric assays measure the absorbance changes related to AST activity, while fluorometric assays provide sensitive detection of AST activity in biological samples.
• Protocol Length and Complexity: Most AST assay kits offer simple and rapid protocols, requiring minimal hands-on time and providing results within a short incubation period.

Applications of AST Assay Kits

• Liver Function Assessment: AST levels are commonly used as a marker for liver health, alongside other enzymes like ALT.
• Disease Diagnosis: Elevated AST levels can indicate conditions such as myocardial infarction, acute pancreatitis, and severe burns.
• Research and Drug Discovery: AST assay kits are valuable tools in studying the effects of drugs on AST activity and in metabolic research.

In summary, AST assay kits are essential tools for measuring AST activity, offering sensitivity, simplicity, and versatility for various research and diagnostic applications.