RT-qPCR mix for probes without ROX

RT-qPCR mix for probes without ROX

Mix for quantitative RT-PCR for probes without ROX are made for use with qPCR devices that do not require this internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations.
The mix for probes consists of a Reverse transcriptase, a Taq polymerase, dNTPs, MgCl2. It is possible to add ROX dye to these mixes for use with qPCR devices requiring the presence of ROX.


Here's how to select the appropriate ROX dye for the qPCR device you are using:

UniversalAll instruments
Without ROXBio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
Low ROXApplied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,
MX3005P, MX3000P
High ROXApplied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;
StepOne, StepOnePlus

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Cat#
Description
Cond.
Price Bef. VAT
NB-54-0396-02
 1000rxns(20ul/rxn) 
NB-54-0396-01
 100rxns(20ul/rxn) 
NB-54-0396-03
 10000rxns(20ul/rxn) 
NB-42-94366-100packs
 100packs