Anti-CTNNB1 antibody (Clone: 4F2)

Clonality:	Monoclonal
Clone ID:	4F2
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Dilution Range:	WB 1:1000-2000 IHC 1:200-500 IF 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CTNNB1
Gene ID:	1499
Uniprot ID:	CTNB1_HUMAN
Specificity:	The antibody detects endogenous Catenin-Beta protein.
Immunogen:	Recombinant Protein of Catenin-Beta

Post Translational Modifications	Phosphorylation at Ser-552 by AMPK promotes stabilization of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. Phosphorylation by SRC at Tyr-333 promotes interaction with isoform M2 of PKM (PKM2).promoting transcription activation. Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. Ubiquitinated and degraded following interaction with SOX9. S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions. O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1. Deacetylated at Lys-49 by SIRT1. Phosphorylated at Thr-556 by herpes virus 1/HHV-1 leading to CTNNB1 inhibition.
Function	Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4. Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts.
Protein Name	Catenin Beta-1 Beta-Catenin
Database Links	Reactome: R-HSA-195253 Reactome: R-HSA-196299 Reactome: R-HSA-201681 Reactome: R-HSA-201722 Reactome: R-HSA-3134973 Reactome: R-HSA-351906 Reactome: R-HSA-3769402 Reactome: R-HSA-381771 Reactome: R-HSA-4086398 Reactome: R-HSA-418990 Reactome: R-HSA-4411364 Reactome: R-HSA-4641262 Reactome: R-HSA-5218920 Reactome: R-HSA-525793 Reactome: R-HSA-5339716 Reactome: R-HSA-5358747 Reactome: R-HSA-5358749 Reactome: R-HSA-5358751 Reactome: R-HSA-5358752 Reactome: R-HSA-5626467 Reactome: R-HSA-8853884 Reactome: R-HSA-8876493 Reactome: R-HSA-8951430 Reactome: R-HSA-9733709 Reactome: R-HSA-9754189 Reactome: R-HSA-9762292 Reactome: R-HSA-9764302 Reactome: R-HSA-9793380 Reactome: R-HSA-9796292 Reactome: R-HSA-9823730 Reactome: R-HSA-9824272 Reactome: R-HSA-9833576
Cellular Localisation	Cytoplasm Nucleus Cytoskeleton Cell Junction Adherens Junction Cell Membrane Microtubule Organizing Center Centrosome Spindle Pole Synapse Cilium Basal Body Colocalized With Rapgef2 And Tjp1 At Cell-Cell Contacts Cytoplasmic When It Is Un-Stable (Highly Phosphorylated) Or Bound To Cdh1 Translocates To The Nucleus When It Is Stabilized (Low Level Of Phosphorylation) Interaction With Glis2 And Muc1 Promotes Nuclear Translocation Interaction With Emd Inhibits Nuclear Localization The Majority Of Beta-Catenin Is Localized To The Cell Membrane In Interphase Colocalizes With Crocc Between Cep250 Puncta At The Proximal End Of Centrioles And This Localization Is Dependent On Crocc And Cep250 In Mitosis When Nek2 Activity Increases It Localizes To Centrosomes At Spindle Poles Independent Of Crocc Colocalizes With Cdk5 In The Cell-Cell Contacts And Plasma Membrane Of Undifferentiated And Differentiated Neuroblastoma Cells Interaction With Fam53b Promotes Translocation To The Nucleus
Alternative Antibody Names	Anti-Catenin Beta-1 antibody Anti-Beta-Catenin antibody Anti-CTNNB1 antibody Anti-CTNNB antibody Anti-OK antibody Anti-SW-cl.35 antibody Anti-PRO2286 antibody

Information sourced from Uniprot.org

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