Anti-MYC antibody (400-454) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.75 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MYC
Gene ID:	4609
Uniprot ID:	MYC_HUMAN
Immunogen Region:	400-454
Immunogen:	Synthetic peptide taken within amino acid region 400-454 on human cMyc protein.

Post Translational Modifications	Phosphorylated by PRKDC. Phosphorylation at Ser-344 by PIM2 leads to the stabilization of MYC. Phosphorylation at Ser-77 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-77 by DYRK2.this primes the protein for subsequent phosphorylation by GSK3B at Thr-73. Phosphorylation at Thr-73 and Ser-77 by GSK3 is required for ubiquitination and degradation by the proteasome. Dephosphorylation at Ser-77 by protein phosphatase 2A (PPP2CA) promotes its degradation.interaction with PPP2CA is enhanced by AMBRA1. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-73 and Ser-77, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRPC4AP) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner.
Function	Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CACGATG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release. Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform.
Protein Name	Myc Proto-Oncogene Protein Class E Basic Helix-Loop-Helix Protein 39 Bhlhe39 Proto-Oncogene C-Myc Transcription Factor P64
Database Links	Reactome: R-HSA-1362277 Reactome: R-HSA-201556 Reactome: R-HSA-2122947 Reactome: R-HSA-2173796 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-4411364 Reactome: R-HSA-5687128 Reactome: R-HSA-5689880 Reactome: R-HSA-6785807 Reactome: R-HSA-69202 Reactome: R-HSA-69656 Reactome: R-HSA-8866911 Reactome: R-HSA-8951430 Reactome: R-HSA-9018519 Reactome: R-HSA-9616222 Reactome: R-HSA-9818749
Cellular Localisation	Nucleus Nucleoplasm Nucleolus
Alternative Antibody Names	Anti-Myc Proto-Oncogene Protein antibody Anti-Class E Basic Helix-Loop-Helix Protein 39 antibody Anti-Bhlhe39 antibody Anti-Proto-Oncogene C-Myc antibody Anti-Transcription Factor P64 antibody Anti-MYC antibody Anti-BHLHE39 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance