Anti-NEK2 antibody (1-100) (Clone: S4MR)

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	NEK2
Gene ID:	4751
Uniprot ID:	NEK2_HUMAN
Immunogen Region:	1-100
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NEK2 (P51955).
Immunogen Sequence:	MPSRAEDYEVLYTIGTGSYG RCQKIRRKSDGKILVWKELD YGSMTEAEKQMLVSEVNLLR ELKHPNIVRYYDRIIDRTNT TLYIVMEYCEGGDLASVITK

Tissue Specificity	Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.
Post Translational Modifications	Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.
Function	Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC.SGO1.NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction. Phosphorylates CCDC102B either directly or indirectly which causes CCDC102B to dissociate from the centrosome and allows for centrosome separation. Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.
Protein Name	Serine/Threonine-Protein Kinase Nek2 Hspk 21 Never In Mitosis A-Related Kinase 2 Nima-Related Protein Kinase 2 Nima-Like Protein Kinase 1
Database Links	Reactome: R-HSA-179409 Reactome: R-HSA-2565942 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-5620912 Reactome: R-HSA-8854518
Cellular Localisation	Isoform 1: Nucleus Nucleus Nucleolus Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Spindle Pole Chromosome Centromere Kinetochore Stk3/Mst2 And Sav1 Are Required For Its Targeting To The Centrosome Colocalizes With Sgo1 And Mad1l1 At The Kinetochore Not Associated With Kinetochore In The Interphase But Becomes Associated With It Upon The Breakdown Of The Nuclear Envelope Has A Nucleolar Targeting/ Retention Activity Via A Coiled-Coil Domain At The C-Terminal End Isoform 2: Cytoplasm Predominantly Cytoplasmic Isoform 4: Nucleus Predominantly Nuclear
Alternative Antibody Names	Anti-Serine/Threonine-Protein Kinase Nek2 antibody Anti-Hspk 21 antibody Anti-Never In Mitosis A-Related Kinase 2 antibody Anti-Nima-Related Protein Kinase 2 antibody Anti-Nima-Like Protein Kinase 1 antibody Anti-NEK2 antibody Anti-NEK2A antibody Anti-NLK1 antibody

Information sourced from Uniprot.org

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