Anti-p53 antibody (Clone: BP53-12)

Clonality:	Monoclonal
Clone ID:	BP53-12
Conjugation:	Unconjugated
Isotype:	IgG2a Lambda
Formulation:	PBS with 0.02% Sodium Azide.
Purification:	Affinity purified from tissue culture.
Concentration:	Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range:	Flow Cytometry (0, 5-1, 0 µg/million cells in 0, 1 ml). Immunoblotting (1 µg/ml for 2h at RT). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (formalin-fixed: 1-2 µg/ml for 30 min at RT; requires boiling tissue sections in 10mM citrate buffer,
Storage Instruction:	Store for up to 1 year at 2-8°C upon receipt.

Gene Symbol:	TP53
Gene ID:	7157
Uniprot ID:	P53_HUMAN
Immunogen:	A BALB/c mouse was immunized with recombinant human p53 protein. Fusion partner: P3-X63-Ag8.653.

Tissue Specificity	Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
Post Translational Modifications	Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Acetylation of Lys-382 by EP300. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner. Acetylation at Lys-381 increases stability. Deacetylation at Lys-381 by SIRT6 decreases its stability, thereby regulating cell senescence. Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1. Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated by NUAK1 at Ser-15 and Ser-392.was initially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1. It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage. Phosphorylation at Ser-15 is required for interaction with DDX3X and gamma-tubulin. Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A. May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line. Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. Ubiquitinated by TRIM24, RFFL, RNF34 and RNF125, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner. Ubiquitinated by COP1, which leads to proteasomal degradation. Ubiquitination and subsequent proteasomal degradation is negatively regulated by CCAR2. Polyubiquitinated by C10orf90/FATS, polyubiquitination is 'Lys-48'-linkage independent and non-proteolytic, leading to TP53 stabilization. Polyubiquitinated by MUL1 at Lys-24 which leads to proteasomal degradation. Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by KMT5A, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Dimethylation at Lys-370 and Lys-382 diminishes p53 ubiquitination, through stabilizing association with the methyl reader PHF20. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation. Monomethylated at Arg-333 and dimethylated at Arg-335 and Arg-337 by PRMT5.methylation is increased after DNA damage and might possibly affect TP53 target gene specificity. Sumoylated with SUMO1. Sumoylated at Lys-386 by UBC9.
Function	Acts as a tumor suppressor in many tumor types.induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis.the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2.
Protein Name	Cellular Tumor Antigen P53 Antigen Ny-Co-13 Phosphoprotein P53 Tumor Suppressor P53
Database Links	Reactome: R-HSA-111448 Reactome: R-HSA-139915 Reactome: R-HSA-1912408 Reactome: R-HSA-2559580 Reactome: R-HSA-2559584 Reactome: R-HSA-2559585 Reactome: R-HSA-2559586 Reactome: R-HSA-3232118 Reactome: R-HSA-349425 Reactome: R-HSA-390471 Reactome: R-HSA-5620971 Reactome: R-HSA-5628897 Reactome: R-HSA-5689880 Reactome: R-HSA-5689896 Reactome: R-HSA-5693565 Reactome: R-HSA-6785807 Reactome: R-HSA-6796648 Reactome: R-HSA-6803204 Reactome: R-HSA-6803205 Reactome: R-HSA-6803207 Reactome: R-HSA-6803211 Reactome: R-HSA-6804114 Reactome: R-HSA-6804115 Reactome: R-HSA-6804116 Reactome: R-HSA-6804754 Reactome: R-HSA-6804756 Reactome: R-HSA-6804757 Reactome: R-HSA-6804758 Reactome: R-HSA-6804759 Reactome: R-HSA-6804760 Reactome: R-HSA-6811555 Reactome: R-HSA-69473 Reactome: R-HSA-69481 Reactome: R-HSA-69541 Reactome: R-HSA-69895 Reactome: R-HSA-8852276 Reactome: R-HSA-8853884 Reactome: R-HSA-8941855 Reactome: R-HSA-8943724 Reactome: R-HSA-9723905 Reactome: R-HSA-9758274 Reactome: R-HSA-983231
Cellular Localisation	Cytoplasm Nucleus Pml Body Endoplasmic Reticulum Mitochondrion Matrix Cytoskeleton Microtubule Organizing Center Centrosome Recruited Into Pml Bodies Together With Chek2 Translocates To Mitochondria Upon Oxidative Stress Translocates To Mitochondria In Response To Mitomycin C Treatment Isoform 1: Nucleus Predominantly Nuclear But Localizes To The Cytoplasm When Expressed With Isoform 4 Isoform 2: Nucleus Localized Mainly In The Nucleus With Minor Staining In The Cytoplasm Isoform 3: Nucleus Localized In The Nucleus In Most Cells But Found In The Cytoplasm In Some Cells Isoform 4: Nucleus Predominantly Nuclear But Translocates To The Cytoplasm Following Cell Stress Isoform 7: Nucleus Isoform 8: Nucleus Localized In Both Nucleus And Cytoplasm In Most Cells In Some Cells Forms Foci In The Nucleus That Are Different From Nucleoli Isoform 9: Cytoplasm
Alternative Antibody Names	Anti-Cellular Tumor Antigen P53 antibody Anti-Antigen Ny-Co-13 antibody Anti-Phosphoprotein P53 antibody Anti-Tumor Suppressor P53 antibody Anti-TP53 antibody Anti-P53 antibody

Information sourced from Uniprot.org

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