Anti-SPHK1 antibody (50-100) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.64-0.72 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:200 IHC: 1:100
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	Sphk1
Gene ID:	20698
Uniprot ID:	SPHK1_MOUSE
Immunogen Region:	50-100
Immunogen:	Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein.

Tissue Specificity	Widely expressed. Expressed in brain (at protein level). Detected in neurons.
Function	Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
Protein Name	Sphingosine Kinase 1 Sk 1 Spk 1 Acetyltransferase Sphk1
Database Links	Reactome: R-MMU-1660661 Reactome: -MMU-390471 Reactome: -MMU-5218921 Reactome: -MMU-9009391
Cellular Localisation	Cytoplasm Endosome Membrane Peripheral Membrane Protein Nucleus Cell Membrane Synapse Translocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent Manner Binds To Membranes Containing Negatively Charged Lipids But Not Neutral Lipids Recruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion
Alternative Antibody Names	Anti-Sphingosine Kinase 1 antibody Anti-Sk 1 antibody Anti-Spk 1 antibody Anti-Acetyltransferase Sphk1 antibody Anti-Sphk1 antibody Anti-Sk1 antibody

Information sourced from Uniprot.org

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