General Info
Host: | Rabbit |
Applications: | WB/ELISA |
Reactivity: | Human/Mouse/Monkey |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Tight junction protein ZO-3 is suitable for use in Western Blot and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
Gene Symbol: | TJP3 |
Gene ID: | 27134 |
Uniprot ID: | ZO3_HUMAN |
Specificity: | ZO3 Polyclonal Antibody detects endogenous levels of protein. |
Immunogen: | Synthesized peptide derived from part of the human protein |
Description
Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions. Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition. With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment. |
Protein Name | Tight Junction Protein Zo-3 Tight Junction Protein 3 Zona Occludens Protein 3 Zonula Occludens Protein 3 |
Cellular Localisation | Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Junction Tight Junction Nucleus Exhibits Predominant Nuclear Expression In Proliferating Cells But Is Exclusively Junctionally Expressed After Confluence Is Reached Shows An Epithelial-Specific Tight Junction Localization In A Tjp1/Tjp2-Dependent Fashion |
Alternative Antibody Names | Anti-Tight Junction Protein Zo-3 antibody Anti-Tight Junction Protein 3 antibody Anti-Zona Occludens Protein 3 antibody Anti-Zonula Occludens Protein 3 antibody Anti-TJP3 antibody Anti-ZO3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance