The NeoPRO Femto Western ECL Substrate, developed by Neo Biotech, is a luminol-based enhanced chemiluminescent substrate that demonstrates exceptional sensitivity and compatibility for immunoblots using horseradish peroxidase (HRP) – conjugated secondary antibodies. This product enables the detection of antigens in the mid-femtogram to the high-femtogram range, thanks to its superior sensitivity and prolonged signal duration. Moreover, the NeoPRO Femto Western ECL Substrate's extended chemiluminescent signal duration allows for imaging using digital and film-based techniques without signal loss. For optimal signal intensity and duration, we recommend employing appropriate dilutions of primary and secondary antibodies.