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Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to α-synuclein, CH22139, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to α-synuclein, CH22139, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies.
Western blot analysis of different tissue lysates using chicken pAb to α-synuclein, CH22139, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.
Western blot analysis of different tissue lysates using chicken pAb to α-synuclein, CH22139, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.