Anti-TIA1 antibody (50-100) {FITC}

Clonality:	Polyclonal
Conjugation:	FITC
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.75 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IHC: 1:50 ICC: 1:50 IF: 1:50 CFM: 1:50
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	TIA1
Gene ID:	7072
Uniprot ID:	TIA1_HUMAN
Immunogen Region:	50-100
Immunogen:	Synthetic peptide taken within amino acid region 50-100 on human TIA1 protein.

Tissue Specificity	Expressed in heart, small intestine, kidney, liver, lung, skeletal muscle, testes, pancreas, and ovary (at protein level).
Post Translational Modifications	Phosphorylated by FASTK.phosphorylation occurs after FAS ligation in FAS-mediated apoptosis and before DNA fragmentation.
Function	RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences. Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing. Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner.this binding is optimal if a 5' splice site is adjacent to IAS1. Activates the use of heterologous 5' splice sites.the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence. By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP. Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIAR mRNA. Acts as a modulator of alternative splicing for the apoptotic FAS receptor, thereby promoting apoptosis. Binds to the 5' splice site region of FAS intron 5 to promote accumulation of transcripts that include exon 6 at the expense of transcripts in which exon 6 is skipped, thereby leading to the transcription of a membrane-bound apoptotic FAS receptor, which promotes apoptosis. Binds to a conserved AU-rich cis element in COL2A1 intron 2 and modulates alternative splicing of COL2A1 exon 2. Also binds to the equivalent AT-rich element in COL2A1 genomic DNA, and may thereby be involved in the regulation of transcription. Binds specifically to a polypyrimidine-rich controlling element (PCE) located between the weak 5' splice site and the intronic splicing silencer of CFTR mRNA to promote exon 9 inclusion, thereby antagonizing PTB1 and its role in exon skipping of CFTR exon 9. Involved in the repression of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs), including target ARE-bearing mRNAs encoding TNF and PTGS2. Also participates in the cellular response to environmental stress, by acting downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SGs), leading to stress-induced translational arrest. Formation and recruitment to SGs is regulated by Zn(2+). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. Isoform Short: Displays enhanced splicing regulatory activity compared with TIA isoform Long.
Protein Name	Cytotoxic Granule Associated Rna Binding Protein Tia1 Nucleolysin Tia-1 Isoform P40 Rna-Binding Protein Tia-1 T-Cell-Restricted Intracellular Antigen-1 Tia-1 P40-Tia-1
Database Links	Reactome: R-HSA-6803529
Cellular Localisation	Nucleus Cytoplasm Stress Granule Accumulates In Cytoplasmic Stress Granules (Sg) Following Cellular Damage Recruitment To Sg Is Induced By Zn(2+)
Alternative Antibody Names	Anti-Cytotoxic Granule Associated Rna Binding Protein Tia1 antibody Anti-Nucleolysin Tia-1 Isoform P40 antibody Anti-Rna-Binding Protein Tia-1 antibody Anti-T-Cell-Restricted Intracellular Antigen-1 antibody Anti-Tia-1 antibody Anti-P40-Tia-1 antibody Anti-TIA1 antibody

Information sourced from Uniprot.org

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