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> Anti-TUBB antibody (Clone: 5G3) {HRP}

Anti-TUBB antibody (Clone: 5G3) {HRP}

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Referencia NB-22-7474-50

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  • Immunofluorescence analysis of Rat-kidney tissue. 1, Beta-tubulin (HRP Conjugated) monoclonal antibody (5G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of Hela, with Beta-tubulin-HRP Conjugated diluted at 1:10, 000, 20, 000, 40, 000, 80, 000, 160, 000, 320, 000.
  • Immunofluorescence analysis of Mouse-testis tissue. 1, Beta-tubulin (HRP Conjugated) monoclonal antibody (5G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-lung-cancer tissue. 1, Beta-tubulin (HRP Conjugated) monoclonal antibody (5G3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Beta-tubulin (HRP Conjugated) monoclonal antibody (5G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Beta-tubulin (HRP Conjugated) monoclonal antibody (5G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Beta-tubulin (HRP Conjugated) monoclonal antibody (5G3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

General Info

Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Tubulin beta chain is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 5G3
Conjugation: HRP
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-1:3000
IHC 1:50-1:200
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: TUBB
Gene ID: 203068
Uniprot ID: TBB5_HUMAN
Specificity: The Beta-tubulin antibody can detects endogenous Beta-tubulin protein.
Immunogen: Recombinant Protein of Beta-tubulin (HRP Conjugated)

Description

Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name Tubulin Beta Chain
Tubulin Beta-5 Chain
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-6798695
Reactome: R-HSA-8854518
Reactome: R-HSA-9679191
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tubulin Beta Chain antibody
Anti-Tubulin Beta-5 Chain antibody
Anti-TUBB antibody
Anti-TUBB5 antibody
Anti-OK antibody
Anti-SW-cl.56 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance