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Rat spinal cord section, stained with CH22134, dilution 1:1,000, in red. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Nuclear DNA is shown with DAPI stain in blue.
Rat spinal cord section, stained with CH22134, dilution 1:1,000, in red. Microglia are very small cells with fine processes spreading in three dimensions and so are best visualized in a confocal Z stack. Nuclear DNA is shown with DAPI stain in blue.
Western blot analysis of chicken pAb to IBA1, CH22134, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.
Western blot analysis of chicken pAb to IBA1, CH22134, dilution 1:1,000 in green: [1] protein standard (red) and [2] rat spleen crude homogenate. The band at about 15kDa mark corresponds to IBA1 protein. IBA1 is a relatively minor protein of brain and is much more abundant in spleen, so the 15kDa band is less obvious in CNS lysates.