General Info
| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Chicken/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-PDE4D is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | FITC |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.2 µg/µl |
| Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:150 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Information
| Gene Symbol: | PDE4D |
| Gene ID: | 5144 |
| Uniprot ID: | PDE4D_HUMAN |
| Immunogen: | Synthetic peptide corresponding to unique amino acid sequence on PDE4D8 variant gene. |
Description
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Protein Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4d Dpde3 Pde43 Camp-Specific Phosphodiesterase 4d |
| Database Links | Reactome: R-HSA-180024 Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell Membrane Cytoplasm Membrane Cytoskeleton Microtubule Organizing Center Centrosome Found In The Soluble Fraction Associated With Membranes And Associated With The Cytoskeleton And The Centrosome Colocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Antibody Names | Anti-3' -5'-Cyclic-Amp Phosphodiesterase 4d antibody Anti-Dpde3 antibody Anti-Pde43 antibody Anti-Camp-Specific Phosphodiesterase 4d antibody Anti-PDE4D antibody Anti-DPDE3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance