Universal RT-qPCR mixes for probes
The universal quantitative RT-PCR mixes for probes are made for use with all types of qPCR devices.
Mixes contain all components for RT-qPCR (Reverse transcriptase,Taq polymerase, dNTPs, MgCl2) and 2 different ROX dyes: High Rox and Low Rox. Simply add DNA template, primers, probes and the corresponding dye for the qPCR device you want to use.
We propose a probe synthesis service for qPCR
The advantage of the universal mixes for probes is therefore to be able to use the same mix for different devices.
Here's how to select the appropriate ROX dye for the qPCR device you are using:
| Universal | All instruments |
| Without ROX | Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler. |
| Low ROX | Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P |
| High ROX | Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus |
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