Cellular Immunology Assays
We propose a set of in vitro efficacy tests using cell assays for immunological studies:
- Immunophenotyping and lymphocyte assays: Assays in whole blood, PBMCs and purified immune cell subsets.
- Toxicity tests-ADCC, ADCP, CDC: Antibody Dependent Cell Cytotoxicity (ADCC) and Complement Dependent Cytotoxicity (CDC) tests.
- Fc receptor binding
- Mixed lymphocyte reactions: Mixed lymphocyte reactions (MLR) are a common method for assessing the cellular immune response.
- Macrophage, Treg and NK cell assays
- Multiplex cytokine profiling
- ELISA and ELISpot: We propose tests with colorimetric, fluorescent, temporal resolution and homogeneous ELISA formats (not requiring washing). ELISpot assays are highly sensitive immunoassays that measure the frequency of cells secreting individual cytokines.
- Immune Function Assays: These assays are performed in human whole blood, purified PBMC or subsets and mouse splenocytes or whole blood. Whole blood provides a particularly useful format for pharmacodynamic dosages.
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ELISA and ELISPOT Assays
Tests with colorimetric, fluorescent, temporal resolution and homogeneous ELISA formats (not requiring washing). ELISpot assays are highly sensitive immunoassays that measure the frequency of cells secreting individual cytokines.
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Cytotoxic T Lymphocyte (CTL) & Natural Killer Activity Assays
CTL assays are commonly used to quantify cytotoxicity
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Mixed Lymphocyte Reaction (MLR)
Mixed lymphocyte reactions (MLR) are a common method to assess the cellular immune response
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Effector Function - Cell Cytotoxicity Assays
Antibody Dependent Cell Cytotoxicity (ADCC) and Complement Dependent Cytotoxicity (CDC) tests
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In vitro efficacity testing - Cell Activation
Assays to measure immune cell activation using multiple readouts, alone or in combination
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Immunophenotyping & Lymphocyte Subset Characterization
Assays in whole blood, PBMCs and purified immune cell subsets
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