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> Anti-CYCS antibody (Clone: 4B10)

Anti-CYCS antibody (Clone: 4B10)

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Referencia NB-22-7416-50

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  • Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS monoclonal antibody.
  • Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
  • Immunofluorescence analysis of Rat-liver tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue using CYCS monoclonal antibody.
  • Immunofluorescence analysis of Human-liver-cancer tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Mouse-spleen tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of lysates from 1) Hela, 2) 293T, 3) 3T3, cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH Polyclonal Antibody (cat: antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host: Mouse
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat/Chicken
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cytochrome c is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 4B10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Dilution Range: WB 1:1000-5000
IHC 1:500-1000
IF 1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: CYCS
Gene ID: 54205
Uniprot ID: CYC_HUMAN
Specificity: The antibody detects endogenous CYCS protein.
Immunogen: Recombinant Protein of CYCS

Description

Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Protein Name Cytochrome C
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-111458
Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-2151201
Reactome: R-HSA-3299685
Reactome: R-HSA-5620971
Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9627069
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Intermembrane Space
Loosely Associated With The Inner Membrane
Alternative Antibody Names Anti-Cytochrome C antibody
Anti-CYCS antibody
Anti-CYC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance