Anti-HLA-G antibody (Clone: MEM-G/11) {FITC}

Clonality:	Monoclonal
Clone ID:	MEM-G/11
Conjugation:	FITC
Isotype:	IgG1
Formulation:	Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration:	1 mg/mL
Storage Instruction:	Store at 2-8°C upon receipt.

Gene Symbol:	HLA-G
Gene ID:	3135
Uniprot ID:	HLAG_HUMAN
Immunogen:	Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Post Translational Modifications	N-glycosylated. Soluble HLA class I histocompatibility antigen, alpha chain G: Produced by proteolytic cleavage at the cell surface (shedding) by matrix metalloproteinase MMP2.
Function	Isoform 1: Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface. In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins. Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance. Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling. Through interaction with LILRB2 receptor triggers differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells. Reprograms B cells toward an immune suppressive phenotype via LILRB1. May induce immune activation/suppression via intercellular membrane transfer (trogocytosis), likely enabling interaction with KIR2DL4, which resides mostly in endosomes. Through interaction with the inhibitory receptor CD160 on endothelial cells may control angiogenesis in immune privileged sites. Isoform 2: Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity. Isoform 3: Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity. Isoform 4: Likely does not bind B2M and presents peptides. Negatively regulates NK cell- and CD8+ T cell-mediated cytotoxicity. Isoform 5: Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface. In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins. Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance. Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling. Through interaction with LILRB2 receptor triggers differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Reprograms B cells toward an immune suppressive phenotype via LILRB1. Isoform 6: Likely does not bind B2M and presents peptides. Isoform 7: Likely does not bind B2M and presents peptides.
Protein Name	Hla Class I Histocompatibility Antigen - Alpha Chain G Hla G Antigen Mhc Class I Antigen G Cleaved Into - Soluble Hla Class I Histocompatibility Antigen - Alpha Chain G Shla-G
Database Links	Reactome: R-HSA-1236974 Reactome: R-HSA-1236977 Reactome: R-HSA-198933 Reactome: R-HSA-877300 Reactome: R-HSA-909733 Reactome: R-HSA-9705671 Reactome: R-HSA-983170
Cellular Localisation	Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Membrane Early Endosome Membrane Soluble Hla Class I Histocompatibility Antigen Alpha Chain G: Secreted Isoform 2: Cell Membrane Isoform 3: Cell Membrane Isoform 4: Cell Membrane Isoform 5: Secreted Early Endosome Isoform 6: Secreted Isoform 7: Secreted Cell Projection Filopodium Membrane Hla-G Trogocytosis From Extravillous Trophoblast's Filopodia Occurs In The Majority Of Decidual Nk Cells
Alternative Antibody Names	Anti-Hla Class I Histocompatibility Antigen - Alpha Chain G antibody Anti-Hla G Antigen antibody Anti-Mhc Class I Antigen G Cleaved Into - Soluble Hla Class I Histocompatibility Antigen - Alpha Chain G antibody Anti-Shla-G antibody Anti-HLA-G antibody Anti-HLA-6.0 antibody Anti-HLAG antibody

Information sourced from Uniprot.org

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