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> Anti-SOX10 antibody (350-450) (Clone: S5MR)

Anti-SOX10 antibody (350-450) (Clone: S5MR)

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Referencia NB-22-48764-200

embalaje : 200uL(2x100uL)

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  • Western blot analysis of extracts of various cell lines, using SOX10 rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of C6 cells using SOX10 Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SOX10 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SOX10 Rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

General Info

Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SOX10 (350-450) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: SOX10
Gene ID: 6663
Uniprot ID: SOX10_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human SOX10 (P56693).
Immunogen Sequence: VDAKAQVKTETAGPQGPPHY TDQPSTSQIAYTSLSLPHYG SAFPSISRPQFDYSDHQPSG PYYGHSGQASGLYSAFSYMG PSQRPLYTAISDPSPSGPQS H

Description

Tissue Specificity Expressed in fetal brain and in adult brain, heart, small intestine and colon.
Function Transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3. Transcriptional activator of MBP, via binding to the gene promoter.
Protein Name Transcription Factor Sox-10
Database Links Reactome: R-HSA-9619665
Reactome: R-HSA-9764302
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion Outer Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Transcription Factor Sox-10 antibody
Anti-SOX10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance