Anti-ATG10 antibody (121-220) (Clone: S1MR)

Référence NB-22-48750-100

Conditionnement : 100uL

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  • Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ATG10 Rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

General Info

Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ATG10 (121-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: ATG10
Gene ID: 83734
Uniprot ID: ATG10_HUMAN
Immunogen Region: 121-220
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 121-220 of human ATG10 (Q9H0Y0).
Immunogen Sequence: RPLTLKDIWEGVHECYKMRL LQGPWDTITQQEHPILGQPF FVLHPCKTNEFMTPVLKNSQ KINKNVNYITSWLSIVGPVV GLNLPLSYAKATSQDERNVP

Description

Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.
Protein Name Ubiquitin-Like-Conjugating Enzyme Atg10
Autophagy-Related Protein 10
Apg10-Like
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ubiquitin-Like-Conjugating Enzyme Atg10 antibody
Anti-Autophagy-Related Protein 10 antibody
Anti-Apg10-Like antibody
Anti-ATG10 antibody
Anti-APG10L antibody
Anti-PP12616 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance