General Info
Host: | Rabbit |
Applications: | WB/IHC/IF |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit monoclonal antibody anti-LysRS (500-597) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
Clonality: | Monoclonal |
Clone ID: | S4MR |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
Purification: | Affinity purification |
Dilution Range: | WB 1:500-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
Gene Symbol: | KARS1 |
Gene ID: | 3735 |
Uniprot ID: | SYK_HUMAN |
Immunogen Region: | 500-597 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-597 of human LysRS/KARS (Q15046). |
Immunogen Sequence: | TELNDPMRQRQLFEEQAKAK AAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLT DSNNIKEVLLFPAMKPEDKK ENVATTDTLESTTVGTSV |
Description
Post Translational Modifications | Phosphorylated on a serine residue after mast cell stimulation with immunoglobulin E (IgE). |
Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity. (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. |
Protein Name | Lysine--Trna Ligase Lysyl-Trna Synthetase Lysrs |
Database Links | Reactome: R-HSA-2408522 Reactome: R-HSA-379716 Reactome: R-HSA-379726 |
Cellular Localisation | Isoform Cytoplasmic: Cytoplasm Cytosol Cytoplasm Nucleus Cell Membrane Peripheral Membrane Protein Secreted Secretion Is Induced By Tnf-Alpha Cytosolic In Quiescent Mast Cells Translocates Into The Nucleus In Response To Mast Cell Activation By Immunoglobulin E Isoform Mitochondrial: Mitochondrion |
Alternative Antibody Names | Anti-Lysine--Trna Ligase antibody Anti-Lysyl-Trna Synthetase antibody Anti-Lysrs antibody Anti-KARS1 antibody Anti-KARS antibody Anti-KIAA0070 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance