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GAPDH

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Référence NB-26-02010

Conditionnement : 100ul

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Product Details
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescence: 1:2,000; Immunohistochemistry: 1:2,000; Western Blot: 1:30,000
Immunogen: Full length recombinant human GAPDH expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (polar packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Bovine, Porcine, Equine
Entrez:
UniProt:
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, RA22148, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. RA22148 produces diffuse cytoplasmic staining of cells.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with rabbit pAb to GAPDH, RA22148, dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. RA22148 produces diffuse cytoplasmic staining of cells.

Western Blot

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, RA22148, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.

Western Blot

Western blot analysis of different cell cytosolic or nuclear enriched fractions, using rabbit pAb to GAPDH, RA22148, dilution 1:20,000, in red: [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. Strong band at 37kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions.