Anti-CD44 antibody (20-178) (Clone: S8MR)

Clonality:	Monoclonal
Clone ID:	S8MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:1000-1:5000 IHC-P 1:1000-1:5000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CD44
Gene ID:	960
Uniprot ID:	CD44_HUMAN
Immunogen Region:	20-178
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3).
Immunogen Sequence:	AQIDLNITCRFAGVFHVEKN GRYSISRTEAADLCKAFNST LPTMAQMEKALSIGFETCRY GFIEGHVVIPRIHPNSICAA NNTGVYILTSNTSQYDTYCF NASAPPEEDCTSVTDLPNAF DGPITITIVNRDGTRYVQKG EYRTNPEDIYPSNPTDDDV

Tissue Specificity	Detected in fibroblasts and urine (at protein level). Detected in placenta (at protein level). Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Post Translational Modifications	Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated.contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated.activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Function	Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment. Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection. Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases. Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion.
Protein Name	Cd44 Antigen Cdw44 Epican Extracellular Matrix Receptor Iii Ecmr-Iii Gp90 Lymphocyte Homing/Adhesion Receptor Hutch-I Heparan Sulfate Proteoglycan Hermes Antigen Hyaluronate Receptor Phagocytic Glycoprotein 1 Pgp-1 Phagocytic Glycoprotein I Pgp-I Cd Antigen Cd44
Database Links	Reactome: R-HSA-1474228 Reactome: R-HSA-202733 Reactome: R-HSA-216083 Reactome: R-HSA-2160916 Reactome: R-HSA-6798695 Reactome: R-HSA-877300
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Microvillus Secreted Colocalizes With Actin In Membrane Protrusions At Wounding Edges Co-Localizes With Rdx Ezr And Msn In Microvilli Localizes To Cholesterol-Rich Membrane-Bound Lipid Raft Domains
Alternative Antibody Names	Anti-Cd44 Antigen antibody Anti-Cdw44 antibody Anti-Epican antibody Anti-Extracellular Matrix Receptor Iii antibody Anti-Ecmr-Iii antibody Anti-Gp90 Lymphocyte Homing/Adhesion Receptor antibody Anti-Hutch-I antibody Anti-Heparan Sulfate Proteoglycan antibody Anti-Hermes Antigen antibody Anti-Hyaluronate Receptor antibody Anti-Phagocytic Glycoprotein 1 antibody Anti-Pgp-1 antibody Anti-Phagocytic Glycoprotein I antibody Anti-Pgp-I antibody Anti-Cd Antigen Cd44 antibody Anti-CD44 antibody Anti-LHR antibody Anti-MDU2 antibody Anti-MDU3 antibody Anti-MIC4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance