Anti-CD81 antibody (Clone: 1D6)

Clonality:	Monoclonal
Clone ID:	1D6
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	PBS with 0.09% sodium azide
Concentration:	1.0 mg/mL
Storage Instruction:	Store at 2-8°C upon receipt.

Gene Symbol:	CD81
Gene ID:	975
Uniprot ID:	CD81_HUMAN
Immunogen:	OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)

Tissue Specificity	Expressed on B cells (at protein level). Expressed in hepatocytes (at protein level). Expressed in monocytes/macrophages (at protein level). Expressed on both naive and memory CD4-positive T cells (at protein level).
Post Translational Modifications	Not glycosylated. Likely constitutively palmitoylated at low levels. Protein palmitoylation is up-regulated upon coligation of BCR and CD9-C2R-CD81 complexes in lipid rafts.
Function	Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation. Can act both as positive and negative regulator of homotypic or heterotypic cell-cell fusion processes. Positively regulates sperm-egg fusion and may be involved in acrosome reaction. In myoblasts, associates with CD9 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion of mononuclear cell progenitors into osteoclasts in charge of bone resorption. May regulate the compartmentalization of enzymatic activities. In T cells, defines the subcellular localization of dNTPase SAMHD1 and permits its degradation by the proteasome, thereby controlling intracellular dNTP levels. Also involved in cell adhesion and motility. Positively regulates integrin-mediated adhesion of macrophages, particularly relevant for the inflammatory response in the lung. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes. Association with CLDN1 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. (Microbial infection) Involved in SAMHD1-dependent restriction of HIV-1 replication. May support early replication of both R5- and X4-tropic HIV-1 viruses in T cells, likely via proteasome-dependent degradation of SAMHD1. (Microbial infection) Specifically required for Plasmodium falciparum infectivity of hepatocytes, controlling sporozoite entry into hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.
Protein Name	Cd81 Antigen 26 Kda Cell Surface Protein Tapa-1 Target Of The Antiproliferative Antibody 1 Tetraspanin-28 Tspan-28 Cd Antigen Cd81
Database Links	Reactome: R-HSA-198933 Reactome: R-HSA-977606
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Associates With Cldn1 And The Cldn1-Cd81 Complex Localizes To The Basolateral Cell Membrane
Alternative Antibody Names	Anti-Cd81 Antigen antibody Anti-26 Kda Cell Surface Protein Tapa-1 antibody Anti-Target Of The Antiproliferative Antibody 1 antibody Anti-Tetraspanin-28 antibody Anti-Tspan-28 antibody Anti-Cd Antigen Cd81 antibody Anti-CD81 antibody Anti-TAPA1 antibody Anti-TSPAN28 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance