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> Anti-COX4I1 antibody (Clone: 4D11-B3-E8)

Anti-COX4I1 antibody (Clone: 4D11-B3-E8)

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Référence NB-22-30904-50

Conditionnement : 50ug

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  • Western blot detection of COX IV in Goat muscle, CHO-k1, COS7, 3T3, Hela, C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
  • Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution) , followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
  • Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8, 1:50 diluted) , showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer (pH6.0).
  • Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.
  • Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.
  • Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.

General Info

Host: Mouse
Applications: WB/FC/ICC/IP/IHC
Reactivity: Human/Mouse/Rat/Hamster/Goat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Cytochrome c oxidase subunit 4 isoform 1, mitochondrial is suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 4D11-B3-E8
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:150
FCM 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: COX4I1
Gene ID: 1327
Uniprot ID: COX41_HUMAN
Specificity: This antibody detects endogenous levels of COX IV and does not cross-react with related proteins.
Immunogen: A synthetic peptide corresponding to carboxyl terminal residues of human COX IV

Description

Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial
Cytochrome C Oxidase Polypeptide Iv
Cytochrome C Oxidase Subunit Iv Isoform 1
Cox Iv-1
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 4 Isoform 1 - Mitochondrial antibody
Anti-Cytochrome C Oxidase Polypeptide Iv antibody
Anti-Cytochrome C Oxidase Subunit Iv Isoform 1 antibody
Anti-Cox Iv-1 antibody
Anti-COX4I1 antibody
Anti-COX4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance