Anti-FTO antibody (1-100) (Clone: S0MR)

Clonality:	Monoclonal
Clone ID:	S0MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200 IP 1:100-1:500
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	FTO
Gene ID:	79068
Uniprot ID:	FTO_HUMAN
Immunogen Region:	1-100
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FTO (Q9C0B1).
Immunogen Sequence:	MKRTPTAEEREREAKKLRLL EELEDTWLPYLTPKDDEFYQ QWQLKYPKLILREASSVSEE LHKEVQEAFLTLHKHGCLFR DLVRIQGKDLLTPVSRILIG

Tissue Specificity	Ubiquitously expressed, with relatively high expression in adrenal glands and brain.especially in hypothalamus and pituitary. Highly expressed in highly expressed in acute myeloid leukemias (AML) with t(11.1)(q23.3) with KMT2A/MLL1 rearrangements, t(15.7)(q21.21)/PML-RARA, FLT3-ITD, and/or NPM1 mutations.
Function	RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells. Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs. Plays an oncogenic role in a number of acute myeloid leukemias by enhancing leukemic oncogene-mediated cell transformation: acts by mediating m6A demethylation of target transcripts such as MYC, CEBPA, ASB2 and RARA, leading to promote their expression.
Protein Name	Alpha-Ketoglutarate-Dependent Dioxygenase Fto Fat Mass And Obesity-Associated Protein U6 Small Nuclear Rna 2'-O-Methyladenosine-N(6--Demethylase Fto U6 Small Nuclear Rna N(6-Methyladenosine-Demethylase Fto Mrna 2'-O-Methyladenosine-N(6--Demethylase Fto M6a(M-Demethylase Fto Mrna N(6-Methyladenosine Demethylase Fto Trna N1-Methyl Adenine Demethylase Fto
Database Links	Reactome: R-HSA-73943
Cellular Localisation	Nucleus Nucleus Speckle Cytoplasm Localizes Mainly In The Nucleus Where It Is Able To Demethylate N(6)-Methyladenosine (M6a) And N(6) 2'-O-Dimethyladenosine Cap (M6a(M)) In U6 Small Nuclear Rna (Snrna) N(1)-Methyladenine From Trnas And Internal M6a In Mrnas In The Cytoplasm Mediates Demethylation Of M6a And M6a(M) In Mrnas And N(1)-Methyladenine From Trnas
Alternative Antibody Names	Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Fto antibody Anti-Fat Mass And Obesity-Associated Protein antibody Anti-U6 Small Nuclear Rna antibody Anti-2'-O-Methyladenosine-N(6--Demethylase Fto antibody Anti-U6 Small Nuclear Rna N(6-Methyladenosine-Demethylase Fto antibody Anti-Mrna antibody Anti-2'-O-Methyladenosine-N(6--Demethylase Fto antibody Anti-M6a(M-Demethylase Fto antibody Anti-Mrna N(6-Methyladenosine Demethylase Fto antibody Anti-Trna N1-Methyl Adenine Demethylase Fto antibody Anti-FTO antibody Anti-KIAA1752 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance