WESTAR SUPERNOVA is a high performance product with an exceptional signal intensity and stable light output for low femtogram detection level.
The formulation provides a low background for a high signal to noise ratio.
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NeoPRO Femto PLUS Western ECL Substrate
Référence NB-78-00005
Conditionnement : 2x50mLkit
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WESTAR SUPERNOVA
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Gallery
Western blotting detection of HDAC-1 on Hela cell lysate with WESTAR SUPERNOVA and its competitors
Sample: Two-fold dilution series of Hela whole cell lysate (abcam®) from 2.5 μg to 0,039 μg of total protein
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:15000
Secondary antibody: Goat anti-Rabbit IgG HRP (2mg/mL) (abcam®) 1:300000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 120 seconds.
Membrane: Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (Biorad)
Blocking: 2% ECL™ Blocking Agent (GE Healthcare) in PBS-T
Primary antibody: Rabbit-anti Human HDAC-1 (abcam®) 1:15000
Secondary antibody: Goat anti-Rabbit IgG HRP (2mg/mL) (abcam®) 1:300000
Imaging: ImageQuant™ LAS 4000 (GE Healthcare)
Exposure time: 120 seconds.
WESTAR SUPERNOVA Signal Duration Comparison with Competitors
Comparison of signal intensities at time points up to 20 hours post substrate addition.
Exposure time is 120 seconds for each time point (0-1-2-5-8-11 hours).
Exposure time is 120 seconds for each time point (0-1-2-5-8-11 hours).
