Anti-HRAS antibody (139-189)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	1.00-1.25 µg/µl
Dilution Range:	WB: 1:500 ELISA: 1:10, 000 IP: 1:150 IHC: 1:50-1:100
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	HRAS
Gene ID:	3265
Uniprot ID:	RASH_HUMAN
Immunogen Region:	139-189
Immunogen:	Synthetic peptide taken within amino acid region 139-189 on human GTPase HRAS protein.

Tissue Specificity	Widely expressed.
Post Translational Modifications	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated.critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Fatty-acylated at Lys-170. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to inhibit Ras signaling.
Function	Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Protein Name	Gtpase Hras H-Ras-1 Ha-Ras Transforming Protein P21 C-H-Ras P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed
Database Links	Reactome: R-HSA-112412 Reactome: R-HSA-1169092 Reactome: R-HSA-1236382 Reactome: R-HSA-1250196 Reactome: R-HSA-1250347 Reactome: R-HSA-1433557 Reactome: R-HSA-167044 Reactome: R-HSA-171007 Reactome: R-HSA-179812 Reactome: R-HSA-180336 Reactome: R-HSA-186763 Reactome: R-HSA-1963640 Reactome: R-HSA-210993 Reactome: R-HSA-2179392 Reactome: R-HSA-2424491 Reactome: R-HSA-2428933 Reactome: R-HSA-2871796 Reactome: R-HSA-375165 Reactome: R-HSA-3928662 Reactome: R-HSA-442982 Reactome: R-HSA-5218921 Reactome: R-HSA-5621575 Reactome: R-HSA-5637810 Reactome: R-HSA-5654688 Reactome: R-HSA-5654693 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658442 Reactome: R-HSA-5673000 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802953 Reactome: R-HSA-6802955 Reactome: R-HSA-74751 Reactome: R-HSA-8849471 Reactome: R-HSA-8851805 Reactome: R-HSA-9026519 Reactome: R-HSA-9027284 Reactome: R-HSA-9028731 Reactome: R-HSA-9034864 Reactome: R-HSA-9607240 Reactome: R-HSA-9634285 Reactome: R-HSA-9634635 Reactome: R-HSA-9648002 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9753510 Reactome: R-HSA-9753512
Cellular Localisation	Cell Membrane Lipid-Anchor Cytoplasmic Side Golgi Apparatus Golgi Apparatus Membrane The Active Gtp-Bound Form Is Localized Most Strongly To Membranes Than The Inactive Gdp-Bound Form Shuttles Between The Plasma Membrane And The Golgi Apparatus Isoform 2: Nucleus Cytoplasm Perinuclear Region Colocalizes With Rack1 To The Perinuclear Region
Alternative Antibody Names	Anti-Gtpase Hras antibody Anti-H-Ras-1 antibody Anti-Ha-Ras antibody Anti-Transforming Protein P21 antibody Anti-C-H-Ras antibody Anti-P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed antibody Anti-HRAS antibody Anti-HRAS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance