Anti-STAT3 antibody (700-750) {Biotin}

Clonality:	Polyclonal
Conjugation:	Biotin
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.55-0.75 µg/µl
Dilution Range:	WB: 1:500 ELISA: 1:10, 000 IP: 1:200
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	STAT3
Gene ID:	6774
Uniprot ID:	STAT3_HUMAN
Immunogen Region:	700-750
Immunogen:	Synthetic peptide taken within amino acid region 700-750 on human STAT3 protein.

Tissue Specificity	Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells.
Post Translational Modifications	Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6, isoform M2 of PKM (PKM2) or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling. Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated. Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated. (Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.
Function	Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation. May play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion.
Protein Name	Signal Transducer And Activator Of Transcription 3 Acute-Phase Response Factor
Database Links	Reactome: R-HSA-1059683 Reactome: R-HSA-111453 Reactome: R-HSA-1266695 Reactome: R-HSA-1433557 Reactome: R-HSA-1839117 Reactome: R-HSA-186763 Reactome: R-HSA-198745 Reactome: R-HSA-201556 Reactome: R-HSA-2559582 Reactome: R-HSA-2586552 Reactome: R-HSA-2892247 Reactome: R-HSA-390471 Reactome: R-HSA-452723 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-8849474 Reactome: R-HSA-8854691 Reactome: R-HSA-8875791 Reactome: R-HSA-8983432 Reactome: R-HSA-8984722 Reactome: R-HSA-8985947 Reactome: R-HSA-9008059 Reactome: R-HSA-9020933 Reactome: R-HSA-9020956 Reactome: R-HSA-9020958 Reactome: R-HSA-9616222 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674555 Reactome: R-HSA-9680350 Reactome: R-HSA-9701898 Reactome: R-HSA-9705462 Reactome: R-HSA-9707564 Reactome: R-HSA-9725370 Reactome: R-HSA-9725371 Reactome: R-HSA-982772
Cellular Localisation	Cytoplasm Nucleus Shuttles Between The Nucleus And The Cytoplasm Translocated Into The Nucleus Upon Tyrosine Phosphorylation And Dimerization In Response To Signaling By Activated Fgfr1 Fgfr2 Fgfr3 Or Fgfr4 Constitutive Nuclear Presence Is Independent Of Tyrosine Phosphorylation Predominantly Present In The Cytoplasm Without Stimuli Upon Leukemia Inhibitory Factor (Lif) Stimulation Accumulates In The Nucleus The Complex Composed Of Bart And Arl2 Plays An Important Role In The Nuclear Translocation And Retention Of Stat3 Identified In A Complex With Lyn And Pag1
Alternative Antibody Names	Anti-Signal Transducer And Activator Of Transcription 3 antibody Anti-Acute-Phase Response Factor antibody Anti-STAT3 antibody Anti-APRF antibody

Information sourced from Uniprot.org

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