General Info
Host: | Rabbit |
Applications: | ELISA/IHC/IP/WB |
Reactivity: | Chicken/Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-HRAS (139-189) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | FITC |
Isotype: | IgG |
Purification: | Affinity Purified |
Concentration: | 1.00-1.25 µg/µl |
Dilution Range: | WB: 1:500 ELISA: 1:10, 000 IP: 1:150 IHC: 1:50-1:100 |
Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Information
Gene Symbol: | HRAS |
Gene ID: | 3265 |
Uniprot ID: | RASH_HUMAN |
Immunogen Region: | 139-189 |
Immunogen: | Synthetic peptide taken within amino acid region 139-189 on human GTPase HRAS protein. |
Description
Tissue Specificity | Widely expressed. |
Post Translational Modifications | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. S-nitrosylated.critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation. The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Fatty-acylated at Lys-170. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to inhibit Ras signaling. |
Function | Involved in the activation of Ras protein signal transduction. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
Protein Name | Gtpase Hras H-Ras-1 Ha-Ras Transforming Protein P21 C-H-Ras P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed |
Database Links | Reactome: R-HSA-112412 Reactome: R-HSA-1169092 Reactome: R-HSA-1236382 Reactome: R-HSA-1250196 Reactome: R-HSA-1250347 Reactome: R-HSA-1433557 Reactome: R-HSA-167044 Reactome: R-HSA-171007 Reactome: R-HSA-179812 Reactome: R-HSA-180336 Reactome: R-HSA-186763 Reactome: R-HSA-1963640 Reactome: R-HSA-210993 Reactome: R-HSA-2179392 Reactome: R-HSA-2424491 Reactome: R-HSA-2428933 Reactome: R-HSA-2871796 Reactome: R-HSA-375165 Reactome: R-HSA-3928662 Reactome: R-HSA-442982 Reactome: R-HSA-5218921 Reactome: R-HSA-5621575 Reactome: R-HSA-5637810 Reactome: R-HSA-5654688 Reactome: R-HSA-5654693 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658442 Reactome: R-HSA-5673000 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802953 Reactome: R-HSA-6802955 Reactome: R-HSA-74751 Reactome: R-HSA-8849471 Reactome: R-HSA-8851805 Reactome: R-HSA-9026519 Reactome: R-HSA-9027284 Reactome: R-HSA-9028731 Reactome: R-HSA-9034864 Reactome: R-HSA-9607240 Reactome: R-HSA-9634285 Reactome: R-HSA-9634635 Reactome: R-HSA-9648002 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9753510 Reactome: R-HSA-9753512 |
Cellular Localisation | Cell Membrane Lipid-Anchor Cytoplasmic Side Golgi Apparatus Golgi Apparatus Membrane The Active Gtp-Bound Form Is Localized Most Strongly To Membranes Than The Inactive Gdp-Bound Form Shuttles Between The Plasma Membrane And The Golgi Apparatus Isoform 2: Nucleus Cytoplasm Perinuclear Region Colocalizes With Rack1 To The Perinuclear Region |
Alternative Antibody Names | Anti-Gtpase Hras antibody Anti-H-Ras-1 antibody Anti-Ha-Ras antibody Anti-Transforming Protein P21 antibody Anti-C-H-Ras antibody Anti-P21ras Cleaved Into - Gtpase Hras - N-Terminally Processed antibody Anti-HRAS antibody Anti-HRAS1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance