Anti-SREBF2 antibody (640-690) {FITC}

Clonality:	Polyclonal
Conjugation:	FITC
Isotype:	IgG
Purification:	Affinity Purified
Concentration:	0.64-0.72 µg/µl
Dilution Range:	WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:150
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	SREBF2
Gene ID:	6721
Uniprot ID:	SRBP2_HUMAN
Immunogen Region:	640-690
Immunogen:	Synthetic peptide taken within amino acid region 640-690 on human Sterol regulatory element binding protein-2.

Tissue Specificity	Ubiquitously expressed in adult and fetal tissues.
Post Translational Modifications	Sterol regulatory element-binding protein 2: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Cleavage and activation is induced by mediated cholesterol efflux. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Processed sterol regulatory element-binding protein 2: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus.
Function	Sterol regulatory element-binding protein 2: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis. Processed sterol regulatory element-binding protein 2: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates transcription of genes related to cholesterol synthesis pathway.
Protein Name	Sterol Regulatory Element-Binding Protein 2 Srebp-2 Class D Basic Helix-Loop-Helix Protein 2 Bhlhd2 Sterol Regulatory Element-Binding Transcription Factor 2 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 2 Transcription Factor Srebf2
Database Links	Reactome: R-HSA-1655829 Reactome: R-HSA-191273 Reactome: R-HSA-1989781 Reactome: R-HSA-2426168 Reactome: R-HSA-381340 Reactome: R-HSA-9619665
Cellular Localisation	Sterol Regulatory Element-Binding Protein 2: Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein Golgi Apparatus Membrane Cytoplasmic Vesicle Copii-Coated Vesicle Membrane At High Sterol Concentrations The Scap-Srebp Is Retained In The Endoplasmic Reticulum Low Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The Golgi Where It Is Processed Processed Sterol Regulatory Element-Binding Protein 2: Nucleus Transported Into The Nucleus With The Help Of Importin-Beta Dimerization Of The Bhlh Domain Is A Prerequisite For Importin Beta-Dependent Nuclear Import
Alternative Antibody Names	Anti-Sterol Regulatory Element-Binding Protein 2 antibody Anti-Srebp-2 antibody Anti-Class D Basic Helix-Loop-Helix Protein 2 antibody Anti-Bhlhd2 antibody Anti-Sterol Regulatory Element-Binding Transcription Factor 2 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 2 antibody Anti-Transcription Factor Srebf2 antibody Anti-SREBF2 antibody Anti-BHLHD2 antibody Anti-SREBP2 antibody

Information sourced from Uniprot.org

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