Anti-Cd274/PD-L1 antibody (Clone: 29E.2A3) {PE/Cyanine7}

Clonality:	Monoclonal
Clone ID:	29E.2A3
Conjugation:	PE/Cyanine7
Isotype:	IgG2bk
Formulation:	PBS with 0.05% Proclin300, 1% BSA
Storage Instruction:	Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product.

Gene Symbol:	CD274
Gene ID:	29126
Uniprot ID:	PD1L1_HUMAN

Tissue Specificity	Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. Isoform 4: Widely expressed, highest in lung, liver and pituitary and in various peripheral blood cells, including neutrophils and some subtypes of lymphoid and myeloid cells.
Post Translational Modifications	Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation.
Function	Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.
Protein Name	Programmed Cell Death 1 Ligand 1 Pd-L1 Pdcd1 Ligand 1 Programmed Death Ligand 1 Hpd-L1 B7 Homolog 1 B7-H1 Cd Antigen Cd274
Database Links	Reactome: R-HSA-389948 Reactome: R-HSA-9701898
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Early Endosome Membrane Recycling Endosome Membrane Nucleus Associates With Cmtm6 At Recycling Endosomes Where It Is Protected From Being Targeted For Lysosomal Degradation Translocates To The Nucleus In Response To Hypoxia Via Its Interaction With Phosphorylated Stat3 Isoform 1: Cell Membrane Isoform 2: Endomembrane System Isoform 4: Secreted
Alternative Antibody Names	Anti-Programmed Cell Death 1 Ligand 1 antibody Anti-Pd-L1 antibody Anti-Pdcd1 Ligand 1 antibody Anti-Programmed Death Ligand 1 antibody Anti-Hpd-L1 antibody Anti-B7 Homolog 1 antibody Anti-B7-H1 antibody Anti-Cd Antigen Cd274 antibody Anti-CD274 antibody Anti-B7H1 antibody Anti-PDCD1L1 antibody Anti-PDCD1LG1 antibody Anti-PDL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance