Sheep polyclonals production
Use of Sheeps give large volumes of antibody.
=> Quote request
This prestation is realized from peptide, recombinant protein, cDNA (genetic immunization), small organisms, haptens, protein extracts etc…
Standard protocol :
- Peptide design
- Peptide synthesis ou Recombinant protein production
- KLH labeling (or any other carrier) if necessary
- Collection of pre-immune serum
- Immunization of Sheep
- Delivery of immune serum
- Option : Affinity purification against the antigen or protein G
Key numbers :
- Minimal number of animals in the protocol : 1
- Protocol length : 80 days
- Number of injections per animal : 5
- Quantity of antigen per injection : 150 µg - 1 mg
- Number of sample collections per animal : 2
- Final amount of antibodies per animal : 250 - 300 mL
We can develop polyclonal antibodies from other species : Rabbit, Chicken, Guinea Pig, Rat, Mouse, Chinese Hamster, Goat.
Related services available :
=> Gene synthesis
=> Gene cloning
=> Peptide synthesis/Recombinant production
=> Other polyclonals production
=> Monoclonals production
=> Antibody labeling
=> Antibody purification
=> Antibody fragmentation
=> Antibody refolding
=> Antibody validation