Mouse polyclonals production
Use of mice give few antibody volumes however they allow a cheaper production with few antigen consuming.
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This prestation is realized from peptide, recombinant protein, cDNA (genetic immunization), small organisms, haptens, protein extracts etc…
Standard protocol :
- Peptide design
- Peptide synthesis ou Recombinant protein production
- KLH labeling (or any other carrier) if necessary
- Collection of pre-immune serum
- Immunization of Mouse
- Delivery of immune serum
- Option : Affinity purification against the antigen or protein G
Key numbers :
- Minimal number of animals in the protocol : 5
- Protocol length : 35-70 days (depending on protocol)
- Number of injections per animal : 3
- Quantity of antigen per injection : 20-50 µg
- Number of sample collections per animal : 1
- Final amount of antibodies per animal : 0.3-1 mL
We can develop polyclonal antibodies from other species : Rabbit, Chicken, Guinea Pig, Rat, Chinese Hamster, Goat, Sheep.
Related services available :
=> Gene synthesis
=> Gene cloning
=> Peptide synthesis/Recombinant production
=> Other polyclonals production
=> Monoclonals production
=> Antibody labeling
=> Antibody purification
=> Antibody fragmentation
=> Antibody refolding
=> Antibody validation