qPCR for diagnostic

Quantitative PCR (qPCR) also known as real-time quantitative PCR is widely used for diagnostic applications.

 

QPCR is a technique based on the principle of PCR that is able to simultaneously amplify and detect changes in amplicon concentration. The qPCR collects data during PCR amplification using a fluorescence signal emitted by probes specific for the amplified sequence or DNA binding dyes.

In medical biology, the applications of qPCR are very numerous and cover very diverse fields, the most important of which are in clinical microbiology, oncology and in the study of gene expression. Very important applications have also been developed in food microbiology and food processing.

 

Clinical microbiology: detection and quantification of bacterial and fungal pathogens; viral titration

 

Clinical oncology: quantification of fusion transcripts, quantification of oncogenes

 

Gene expression: estimation of the level of expression of genes of interest, analysis of the expression of gene splicing variants and validation of the expression profiles obtained using DNA arrays