General Info
| Host: | Mouse |
| Applications: | Activ/Depletion/Inhibition/FC/IHC |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-T-cell surface glycoprotein CD3 epsilon chain is suitable for use in Universal, Universal, Universal, Flow Cytometry and Immunohistochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | OKT3 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | 0.2 µm filtered in PBS, pH7.2. Azide Free (AF) /Low Endotoxin (LE) : Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as determined by LAL gel clotting assay. |
| Concentration: | 0.5 mg/mL |
| Storage Instruction: | Store at 2-8°C. Do not freeze this product. |
Information
| Gene Symbol: | CD3E |
| Gene ID: | 916 |
| Uniprot ID: | CD3E_HUMAN |
Description
| Post Translational Modifications | Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8. |
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. |
| Protein Name | T-Cell Surface Glycoprotein Cd3 Epsilon ChainT-Cell Surface Antigen T3/Leu-4 Epsilon ChainCd Antigen Cd3e |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-202424Reactome: R-HSA-202427Reactome: R-HSA-202430Reactome: R-HSA-202433Reactome: R-HSA-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd3 Epsilon Chain antibodyAnti-T-Cell Surface Antigen T3/Leu-4 Epsilon Chain antibodyAnti-Cd Antigen Cd3e antibodyAnti-CD3E antibodyAnti-T3E antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance