Anti-MUC1/Polymorphic epithelial mucin antibody (Clone: 2G7)

Clonality:	Monoclonal
Clone ID:	2G7
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	PBS with 0.02% sodium azide
Concentration:	100 ug/mL
Storage Instruction:	Store at 2-8°C upon receipt.

Gene Symbol:	MUC1
Gene ID:	4582
Uniprot ID:	MUC1_HUMAN
Immunogen:	VNTR 60mer glycosylated synthetic peptide BSA conjugate

Tissue Specificity	Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.
Post Translational Modifications	Highly glycosylated (N- and O-linked carbohydrates and sialic acid). O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Further sialylation occurs during recycling. Membrane-shed glycoproteins from kidney and breast cancer cells have preferentially sialyated core 1 structures, while secreted forms from the same tissues display mainly core 2 structures. The O-glycosylated content is overlapping in both these tissues with terminal fucose and galactose, 2- and 3-linked galactose, 3- and 3,6-linked GalNAc-ol and 4-linked GlcNAc predominating. Differentially O-glycosylated in breast carcinomas with 3,4-linked GlcNAc. N-glycosylation consists of high-mannose, acidic complex-type and hybrid glycans in the secreted form MUC1/SEC, and neutral complex-type in the transmembrane form, MUC1/TM. Proteolytic cleavage in the SEA domain occurs in the endoplasmic reticulum by an autoproteolytic mechanism and requires the full-length SEA domain as well as requiring a Ser, Thr or Cys residue at the P + 1 site. Cleavage at this site also occurs on isoform MUC1/X but not on isoform MUC1/Y. Ectodomain shedding is mediated by ADAM17. Dual palmitoylation on cysteine residues in the CQC motif is required for recycling from endosomes back to the plasma membrane. Phosphorylated on tyrosines and serine residues in the C-terminal. Phosphorylation on tyrosines in the C-terminal increases the nuclear location of MUC1 and beta-catenin. Phosphorylation by PKC delta induces binding of MUC1 to beta-catenin/CTNNB1 and thus decreases the formation of the beta-catenin/E-cadherin complex. Src-mediated phosphorylation inhibits interaction with GSK3B. Src- and EGFR-mediated phosphorylation on Tyr-1229 increases binding to beta-catenin/CTNNB1. GSK3B-mediated phosphorylation on Ser-1227 decreases this interaction but restores the formation of the beta-cadherin/E-cadherin complex. On T-cell receptor activation, phosphorylated by LCK. PDGFR-mediated phosphorylation increases nuclear colocalization of MUC1CT and CTNNB1. The N-terminal sequence has been shown to begin at position 24 or 28.
Function	The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
Protein Name	Mucin-1 Muc-1 Breast Carcinoma-Associated Antigen Df3 Cancer Antigen 15-3 Ca 15-3 Carcinoma-Associated Mucin Episialin H23ag Krebs Von Den Lungen-6 Kl-6 Pemt Peanut-Reactive Urinary Mucin Pum Polymorphic Epithelial Mucin Pem Tumor-Associated Epithelial Membrane Antigen Ema Tumor-Associated Mucin Cd Antigen Cd227 Cleaved Into - Mucin-1 Subunit Alpha Muc1-Nt Muc1-Alpha - Mucin-1 Subunit Beta Muc1-Beta Muc1-Ct
Database Links	Reactome: R-HSA-5083625 Reactome: R-HSA-5083632 Reactome: R-HSA-5083636 Reactome: R-HSA-5621480 Reactome: R-HSA-6785807 Reactome: R-HSA-913709 Reactome: R-HSA-977068
Cellular Localisation	Apical Cell Membrane Single-Pass Type I Membrane Protein Exclusively Located In The Apical Domain Of The Plasma Membrane Of Highly Polarized Epithelial Cells After Endocytosis Internalized And Recycled To The Cell Membrane Located To Microvilli And To The Tips Of Long Filopodial Protusions Isoform 5: Secreted Isoform Y: Secreted Isoform 9: Secreted Mucin-1 Subunit Beta: Cell Membrane Cytoplasm Nucleus On Egf And Pdgfrb Stimulation Transported To The Nucleus Through Interaction With Ctnnb1 A Process Which Is Stimulated By Phosphorylation On Hrg Stimulation Colocalizes With Jup/Gamma-Catenin At The Nucleus
Alternative Antibody Names	Anti-Mucin-1 antibody Anti-Muc-1 antibody Anti-Breast Carcinoma-Associated Antigen Df3 antibody Anti-Cancer Antigen 15-3 antibody Anti-Ca 15-3 antibody Anti-Carcinoma-Associated Mucin antibody Anti-Episialin antibody Anti-H23ag antibody Anti-Krebs Von Den Lungen-6 antibody Anti-Kl-6 antibody Anti-Pemt antibody Anti-Peanut-Reactive Urinary Mucin antibody Anti-Pum antibody Anti-Polymorphic Epithelial Mucin antibody Anti-Pem antibody Anti-Tumor-Associated Epithelial Membrane Antigen antibody Anti-Ema antibody Anti-Tumor-Associated Mucin antibody Anti-Cd Antigen Cd227 Cleaved Into - Mucin-1 Subunit Alpha antibody Anti-Muc1-Nt antibody Anti-Muc1-Alpha - Mucin-1 Subunit Beta antibody Anti-Muc1-Beta antibody Anti-Muc1-Ct antibody Anti-MUC1 antibody Anti-PUM antibody

Information sourced from Uniprot.org

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