MMLV Reverse Transcriptase

MMLV Reverse Transcriptase

MMLV reverse transcriptase (or M-MuLV reverse transcriptase) is coded by Moloney murine leukemia virus. MMLV reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes the first complementary DNA strand (cDNA) from a single-stranded RNA model to which a primer has been hybridized. This enzyme is also capable of extending the hybridized primers to single-stranded DNA. The synthesis of the second strand of the cDNA can be obtained from certain mRNA models without additional DNA polymerase. M-MLV reverse transcriptase is used for the preparation of cDNA libraries or for cDNA synthesis by Reverse-Transcription PCR (RT-PCR).
M-MuLV reverse transcriptase lacks 3 '→ 5' exonuclease activity. Compared to AMV reverse transcriptase, MMLV has no endonuclease activity and has a lower RNase H activity. RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyses the phosphodiester bonds of RNA that is hybridized to DNA. This enzyme does not digest single-stranded or double-stranded DNA.

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  • Biochemicals 16
  • pcr products 3
  • PCR 1
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NB-54-0181
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 40000U 
NB-42-94362-50,000U
 50,000U 
NB-42-94362-10,000U
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NB-64-40281-25mg
 25mg 
NB-64-40281-50mg
 50mg 
NB-64-40284-25mg
 25mg 
NB-64-40281-100mg
 100mg 
NB-64-40284-100mg
 100mg 
NB-64-40284-50mg
 50mg 
NB-64-04209-100mg
 100mg 
NB-64-04209-25mg
 25mg 
NB-64-04209-50mg
 50mg 
NB-64-04209-10mg
 10mg 
NB-64-04209-200mg
 200mg