General Info
| Host: | Mouse |
| Applications: | FC/IF/IHC |
| Reactivity: | Cat/Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Keratin 5+14 is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | EBS-IF-001 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2ak |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunofluorescence (1-2 µg/ml). Immunohistology (1-2 µg/ml for 30 min at RT; an appropriate anti |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
Information
| Immunogen: | A hybrid (BALB/c x B6) mouse was immunized with a cytokeratin-enriched extract of feline tongue epithelium. Fusion partner: Sp2/0. |
Description
| Background | EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance