General Info
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.117ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This ARO Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
Information
| Gene Symbol: | Cyp19a1 |
| Gene ID: | 13075 |
| Uniprot ID: | CP19A_MOUSE |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). |
| Protein Name | AromataseCypxixCytochrome P-450aromCytochrome P450 19a1Estrogen Synthase |
| Database Links | Reactome: R-MMU-193144Reactome: -MMU-211976 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative ELISA Names | Aromatase ELISA kitCypxix ELISA kitCytochrome P-450arom ELISA kitCytochrome P450 19a1 ELISA kitEstrogen Synthase ELISA kitCyp19a1 ELISA kitArom ELISA kitCyp19 ELISA kit |
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Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance