Anti-BECN1 antibody (Clone: 4F4-B10-C9)

Clonality:	Monoclonal
Clone ID:	4F4-B10-C9
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:1000 IP 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	BECN1
Gene ID:	8678
Uniprot ID:	BECN1_HUMAN
Specificity:	This antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins.
Immunogen:	Purified recombinant human Beclin 1 protein fragments expressed in E.coli.

Post Translational Modifications	Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy. Polyubiquitinated by NEDD4, both with 'Lys-11'- and 'Lys-63'-linkages. 'Lys-11'-linked polyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Polyubiquitinated at Lys-402 with 'Lys-48'-linkages. 'Lys-48'-linked polyubiquitination of Lys-402 leads to degradation. Deubiquitinated by ATXN3, leading to stabilization. Ubiquitinated at Lys-437 via 'Lys-63'-linkage by the DCX(AMBRA1) complex, thereby increasing the association between BECN1 and PIK3C3 to promote PIK3C3 activity. 'Lys-48'-linked ubiquitination by RNF216 leads to proteasomal degradation and autophagy inhibition. Proteolytically processed by caspases including CASP8 and CASP3.the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.
Function	Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate.different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis.it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Protein Name	Beclin-1 Coiled-Coil Myosin-Like Bcl2-Interacting Protein Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda
Database Links	Reactome: R-HSA-1632852 Reactome: R-HSA-5689880 Reactome: R-HSA-9679504 Reactome: R-HSA-9694676 Reactome: R-HSA-9705671 Reactome: R-HSA-983170
Cellular Localisation	Cytoplasm Golgi Apparatus Trans-Golgi Network Membrane Peripheral Membrane Protein Endosome Membrane Endoplasmic Reticulum Membrane Mitochondrion Membrane Endosome Cytoplasmic Vesicle Autophagosome Interaction With Atg14 Promotes Translocation To Autophagosomes Expressed In Dendrites And Cell Bodies Of Cerebellar Purkinje Cells Beclin-1-C 35 Kda: Mitochondrion Nucleus Beclin-1-C 37 Kda: Mitochondrion
Alternative Antibody Names	Anti-Beclin-1 antibody Anti-Coiled-Coil Myosin-Like Bcl2-Interacting Protein antibody Anti-Protein Gt197 Cleaved Into - Beclin-1-C 35 Kda - Beclin-1-C 37 Kda antibody Anti-BECN1 antibody Anti-GT197 antibody

Information sourced from Uniprot.org

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