General Info
| Host: | Rat |
| Applications: | FC |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: | Rat monoclonal antibody anti-Programmed cell death protein 1 is suitable for use in Flow Cytometry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | 29F.1A12 |
| Conjugation: | AF647 |
| Isotype: | IgG2ak |
| Formulation: | PBS with 0.05% Proclin300, 1% BSA |
| Concentration: | 0.5 mg/mL |
| Storage Instruction: | Recommend storing between 2-8°C and protecting from prolonged exposure to light. Do not freeze this product. |
Information
| Gene Symbol: | Pdcd1 |
| Gene ID: | 18566 |
| Uniprot ID: | PDCD1_MOUSE |
Description
| Tissue Specificity | Thymus-specific. |
| Post Translational Modifications | Ubiquitinated at Lys-235 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains. Tyrosine phosphorylated at Tyr-225 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. |
| Protein Name | Programmed Cell Death Protein 1Protein Pd-1Mpd-1Cd Antigen Cd279 |
| Database Links | Reactome: R-MMU-389948 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Programmed Cell Death Protein 1 antibodyAnti-Protein Pd-1 antibodyAnti-Mpd-1 antibodyAnti-Cd Antigen Cd279 antibodyAnti-Pdcd1 antibodyAnti-Pd1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance