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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CYCS antibody (Clone: 4B10)

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Cat# NB-22-7416-50

Size : 50ug

Contact local distributor :

Phone : +1 850 650 7790

Contact local distributor :

Phone : +1 850 650 7790

Immunohistochemical analysis of paraffin-embedded human Breast caricnoma using CYCS monoclonal antibody.
Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
Immunofluorescence analysis of Rat-liver tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue using CYCS monoclonal antibody.
Immunofluorescence analysis of Human-liver-cancer tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-spleen tissue. 1, CYCS monoclonal antibody (4B10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CYCS monoclonal antibody (4B10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from 1) Hela, 2) 293T, 3) 3T3, cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH Polyclonal Antibody (cat: antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS monoclonal antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Mouse
Applications:	WB/IF/IHC
Reactivity:	Human/Mouse/Rat/Chicken
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Cytochrome c is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	4B10
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Dilution Range:	WB 1:1000-5000 IHC 1:500-1000 IF 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	CYCS
Gene ID:	54205
Uniprot ID:	CYC_HUMAN
Specificity:	The antibody detects endogenous CYCS protein.
Immunogen:	Recombinant Protein of CYCS

Description

Function	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Protein Name	Cytochrome C
Database Links	Reactome: R-HSA-111457 Reactome: R-HSA-111458 Reactome: R-HSA-111459 Reactome: R-HSA-111463 Reactome: R-HSA-111464 Reactome: R-HSA-2151201 Reactome: R-HSA-3299685 Reactome: R-HSA-5620971 Reactome: R-HSA-5628897 Reactome: R-HSA-611105 Reactome: R-HSA-9627069 Reactome: R-HSA-9707564
Cellular Localisation	Mitochondrion Intermembrane Space Loosely Associated With The Inner Membrane
Alternative Antibody Names	Anti-Cytochrome C antibody Anti-CYCS antibody Anti-CYC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance