Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-SARS-CoV-2 Spike S1 antibody (11-682)

Anti-SARS-CoV-2 Spike S1 antibody (11-682)

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Cat# NB-22-48212-200

Size : 200uL(2x100uL)

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Phone : +1 850 650 7790

  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike S1 antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S1 antibody at a dilution of 1:10000.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S1 fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S1 Rabbit polyclonal antibody at dilution of 1:400 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immobilized Recombinant SARS-COV-2 Spike S1 Protein (RP01262LQ) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike S1 Rabbit polyclonal antibody with a linear range of 0. 78-50ng/mL.
  • Western blot analysis of lysates from 293T cells, using SARS-CoV-2 Spike S1 Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

General Info

Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2 Spike S1 (11-682) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
ELISA 1:20000-1:80000
WB 1:500-1:1000
IF/ICC 1:100-1:500
IP 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 11-682
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 11-682 of coronavirus Spike S1 (YP_009724390.1).
Immunogen Sequence: VSSQCVNLTTRTQLPPAYTN SFTRGVYYPDKVFRSSVLHS TQDLFLPFFSNVTWFHAIHV SGTNGTKRFDNPVLPFNDGV YFASTEKSNIIRGWIFGTTL DSKTQSLLIVNNATNVVIKV CEFQFCNDPFLGVYYHKNNK SWMESEFRVYSSANNCTFEY VSQPFLMDLEGKQGNFKNLR EFVFKNIDGYFKIYSKHTPI NLVRDLPQGFSALEPLVDLP IGINITRFQTLLALHRSYL

Description

Background This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance