IL-13, Anti-Murine
Cat# NB-50-0791-50
Size : 50µg
Contact local distributor :
Phone : +1 850 650 7790
Product Image Gallery
Western Blot: To detect Murine IL-13 by Western Blot analysis IL-13 Polyclonal Antibody (Product # 500-P178-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Western Blot: To detect Murine IL-13 by Western Blot analysis IL-13 Polyclonal Antibody (Product # 500-P178-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA: To detect Murine IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-13 Polyclonal Antibody (Product # 500-P178-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-13 Polyclonal Antibody, Biotin (Product # 500-P178BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-13.
Product Details
500-P178-100UG
Species Reactivity
Published species
Host/Isotype
Class
Type
Immunogen
Conjugate
Form
Concentration
Purification
Storage buffer
Contains
Storage conditions
Shipping conditions
RRID
Product Specific Information
AA Sequence of recombinant protein: MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-13. Anti-Murine IL-13-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-13 matrix.
Sandwich ELISA: To detect Murine IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-13 (500-P178Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-13.
Western Blot: To detect Murine IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.