Anti-BDNF antibody (Clone: 4C8)

Clonality:	Monoclonal
Clone ID:	4C8
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	PBS with 0.1% sodium azide
Purification:	Protein G purification
Dilution Range:	ELISA 0.05 to 0.1 Mu g/ml; WB 0.2 to 1 Mu g/ml; IF 20 Mu g/ml (weak signal)
Storage Instruction:	Store at-20 to-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Gene Symbol:	BDNF
Gene ID:	627
Uniprot ID:	BDNF_HUMAN
Specificity:	Human, mouse, rat. guinea pig
Immunogen:	Recombinant human BDNF protein purified from E. coli. Human BDNF

Tissue Specificity	Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
Post Translational Modifications	BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG).
Function	Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
Protein Name	Brain-Derived Neurotrophic Factor Bdnf Abrineurin Cleaved Into - Bdnf Precursor Form Probdnf
Database Links	Reactome: R-HSA-9022702 Reactome: R-HSA-9024909 Reactome: R-HSA-9026519 Reactome: R-HSA-9026527 Reactome: R-HSA-9028335 Reactome: R-HSA-9028731 Reactome: R-HSA-9032500 Reactome: R-HSA-9032759 Reactome: R-HSA-9032845 Reactome: R-HSA-9768919
Cellular Localisation	Secreted Bdnf Precursor Form: Secreted A Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf)
Alternative Antibody Names	Anti-Brain-Derived Neurotrophic Factor antibody Anti-Bdnf antibody Anti-Abrineurin Cleaved Into - Bdnf Precursor Form antibody Anti-Probdnf antibody Anti-BDNF antibody

Information sourced from Uniprot.org

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