Mouse CSF-1 receptor (CSF-1R) protein (Recombinant)

Conjugation:	Unconjugated
Formulation:	Lyophilised 0.2 Mu m filtered PBS solution, pH7.2.
Storage Instruction:	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Immunoreactivity:	Activity of recombinant mouse CSF-1 receptor was measured via its ability to bind biotinylated recombinant human M-SCF. The ED50 of biotinylated human M-CSF is 0.5 ug/mL NA

Gene Symbol:	Csf1r
Gene ID:	12978
Uniprot ID:	CSF1R_MOUSE
Immunogen Region:	ECD
Immunogen:	Optimized DNA sequence encoding extracellular domain of mouse CSF-1 receptor (CSF-1R) including a C-terminal polyHis tag was expressed in HEK293 cells. NA

Tissue Specificity	Widely expressed.
Post Translational Modifications	Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-559 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-559 and Tyr-807 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-697 and Tyr-921 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-721 and Tyr-807 is important for interaction with PLCG2. Phosphorylation at Tyr-974 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation. Ubiquitinated. Becomes rapidly polyubiquitinated after autophosphorylation, leading to its degradation.
Function	Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages.
Protein Name	Macrophage Colony-Stimulating Factor 1 Receptor Csf-1 Receptor Csf-1-R Csf-1r M-Csf-R Proto-Oncogene C-Fms Cd Antigen Cd115
Database Links	Reactome: R-MMU-449836
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein The Autophosphorylated Receptor Is Ubiquitinated And Internalized Leading To Its Degradation
Alternative Protein Names	Macrophage Colony-Stimulating Factor 1 Receptor protein Csf-1 Receptor protein Csf-1-R protein Csf-1r protein M-Csf-R protein Proto-Oncogene C-Fms protein Cd Antigen Cd115 protein Csf1r protein Csfmr protein Fms protein

Information sourced from Uniprot.org

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