Endotoxin removal kits
The endotoxins are small hydrophobic molecules, lipopolysaccharides (LPS), associated with the outer membranes of certain microorganisms and more particularly the Gram-negative bacteria (Brucella, Neisseria, Vibrio ...). These small thermostable molecules are not secreted but are released only when the cells are destroyed. In large quantities, endotoxins can cause haemorrhagic shock and acute diarrhea, lower levels may cause fever, lower resistance to bacterial infections, leukopenia followed by leukocytosis and other biological effects, depending of organism and its physiological conditions.
Small amounts of endotoxins in recombinant protein or antibody production can cause tissue damage and even death of the host organism. Endotoxins should therefore be removed from drugs, injectables and other biological products in order to avoid these effects.
The effects of endotoxins vary from one organism or cell type to another. This is due to the different cellular responses to endotoxins. The critical level of endotoxins is therefore very variable according to the organisms or cell types, which is why it is preferable to have an endotoxin level as low as possible.
Spin columns | NoEndo M | NoEndo S | NoEndo HC |
Typical in situ binding capacity per column | 3,000 EU | 30,000 EU | 500,000-1,000,000 EU |
Typical endotoxin binding capacity | 300 EU/ml | 1,500 EU/ml | 30,000 EU/ml |
Minimum endotoxin levels tested post-column | <0.03 EU/ml | <0.05 EU/ml | <0.05 EU/ml |
Typical endotoxin clearance after 1 pass | - | 3 log reduction | 3 log reduction |
Typical endotoxin clearance after 2 passes | - | 4 log reduction | 4 log reduction |
Typical endotoxin clearance after 1 hour incubation | 2 log reduction | - | - |
Typical endotoxin clearance after 3 hour incubation | 3 log reduction | - | - |
Maximum sample load volume | 20 ml | 20 ml | 20 ml |
Bed volume | 0.25 ml loose | 1 ml pre-packed | 1.7 ml pre-packed |
Resin | NoEndo resin | NoEndo resin | NoEndo resin |
Bead size range | 100 μm | 100 μm | 100 μm |
Proteus matrix | Cross-linked
6 % agarose |
Cross-linked
6 % agarose |
Cross-linked
6 % agarose |
Recommended working pH | 4-8 | 4-8 | 4-8 |
Colour coded end-caps | Supplied in vials | Light green | Dark green |
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